The biotinylated horse anti-goat IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
|Unit Size||1.5 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs|
|Concentration||1.5 mg active conjugate/ml|
|Recommended Storage||2-8 °C; Store frozen for long term storage|
|Solution||10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.|
|Recommended Usage||The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note: The use of bovine products such as casein, serum, albuminor non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. NOTE: This product is raised in horse and is not the same as that contained in the VECTASTAIN ABC Kits for the detection of goat IgG which is raised in rabbit.|
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