Biotinylated Goat Anti-Human Kappa Chain Antibody is a chain-specific antibody that can be used for tissue and cell staining, ELISAs, and blots. This kappa chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.
|Unit Size||0.5 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs|
|Recommended Storage||2–8 °C|
|Recommended Usage||Reconstitute by adding 1.0 ml water. The resulting solution will have the following composition: 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.|
Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques.
Biotinylated Goat Anti-Human Kappa Chain is supplied in solution form. With some exceptions the recommended dilution for most applications is 1:100.
This chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins. This antibody is affinity-purified and treated by negative solid-phase adsorption to remove cross-reacting antibodies. This biotinylated conjugate can be employed in flow cytometry or used for tissue staining.
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