This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
Biotinylated Dolichos biflorus agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
|Unit Size||5 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology|
|Recommended Usage||For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.|
|Recommended Storage||2-8 °C; Store frozen for long term storage|
|Solution||10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.|
|Concentration||5 mg active conjugate/ml|
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
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