Molecular Weight 130 | |
Extinction Coefficient 1.33 | |
Formulation 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide. | |
Inhibiting or Eluting Sugar Neuraminidase treatment or Fetuin | |
Unit Size 1 mg | |
Storage Instructions 2-8 °C | |
Sugar Specificity α2,3-sialylated Galb1−3GalNAc in O-glycans, and 3′O-sulfated Gal. | |
Usage Summary For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology | |
Concentration 1 mg active conjugate/ml | |
Conjugate Biotinylated |
Maackia Amurensis Lectin II (MAL II), Biotinylated
Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike Sambucus nigra lectin (SNA) which seems to prefer structures with (α-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (α-2,3) linkage. Tissue staining patterns are also very different among MAL I, SNA and MAL II.
Biotinylated Maackia amurensis lectin II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
$247.00
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Technical Information Maackia amurensis lectin II (MAH) is a glycoprotein consisting of two subunits each of which is composed of disulfide-linked chains. This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011 Inhibitor: Human glycophorin |
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Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
