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3' EndTag™ DNA End Labeling System

MB-9002
SKU Unit Size Price Qty
MB-9002 1 kit
$252.00
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Description

3' end labeling is the preferred method for oligonucleotide labeling if the terminal phosphate at the 5' end must be preserved.


The 3' EndTag System enables simple and uniform labeling of blunt, overhanging, or recessed 3' ends of DNA. Using this system, a variety of fluorescent dyes, haptens, or affinity tags can be covalently attached to the 3' end using thiol-specific chemistry. Additionally, labels containing thiol-reactive groups (maleimides, iodoacetamides, etc.) can easily be incorporated.

Specifications

More Information
Unit Size 1 kit
Target for Labeling DNA
Required Reactive Group 3' -OH group of DNA
Tag/Group Incorporated Thiol
Safety Title WARNING: NA - www.P65Warnings.ca.gov

Kit Components

What's in the box?

The 3' EndTag Labeling Kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction. This kit includes:

  • Terminal Transferase (TdT)
  • SH-GTP
  • 10x TdT buffer
  • Precipitant

*Note: This kit requires Thiol-reactive labeling reagent, which is not included in the kit and must be purchased separately. Labels suitable for this kit include:

Note regarding shipping: This item is shipped on wet ice and therefore requires additional shipping charges.

Documents

Related Products

Biotin (Long Arm) Maleimide Biotin (Long Arm) Maleimide Catalog #: SP-1501-12
PHOTOPROBE® Biotin for Nucleic Acid Labeling PHOTOPROBE® Biotin for Nucleic Acid Labeling Catalog #: SP-1000
PHOTOPROBE® (Long Arm) Biotin for Nucleic Acid Labeling PHOTOPROBE® (Long Arm) Biotin for Nucleic Acid Labeling Catalog #: SP-1020
A std curve is produced using kit provided material standards. Testing sample is mixed with kit reagents and an absorbance value is taken at 535nm. This value is then compared to the standard curve to determine nmols of biotin present in the sample. QuantTag™ Biotin Quantitation Kit Catalog #: BDK-2000

Citations

Technical Information

End labeling is a preferred method for applications in which an internal label might interfere with hybridization or sequence specific protein binding.

5' or 3' EndTag labeled nucleic acids can be used for applications such as DNA hybridization, PCR, in situ hybridization, the binding of capture probes to affinity matrices, or electrophoretic mobility shift assays (EMSA). Short oligonucleotides are labeled more efficiently with these systems than with other methods. In addition, end labeling of oligonucleotides is an economical alternative to having labels inserted during synthesis.

Labeling time is about 1 hour with very little hands-on time required.

3' EndTag Labeling Kit

Labeling using the 3' EndTag Labeling Kit is achieved in two steps:

  1. Terminal deoxynucleotidyl transferase (TdT) incorporates the thiol-containing nucleotide (SH-GTP) onto the 3' end.
  2. Thiolated DNA is coupled with a thiol-reactive label.

Note: Thiol-reactive label is not included in the kit and must be purchased separately.
3 EndTag System

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