Azido-dPEG®₃₆-OH

Azido-dPEG®36-OH (Azido-dPEG®36-alcohol), product number QBD-10544, is a click chemistry reagent used to modify surfaces and biomolecules or to crosslink with other surfaces or biomolecules in supramolecular construction. This product consists of an azide and a terminal hydroxy group separated by a long (108 atoms), single molecular weight, discrete-length polyethylene glycol (dPEG®) chain. The terminal azide reacts in copper(I)-catalyzed, ruthenium-catalyzed, and strain-promoted azide-alkyne cycloaddition reactions (CuAAC, RuAAC, and SPAAC, respectively). If left unmodified, the alcohol group increases the water solubility and hydrodynamic volume of molecules and surfaces to which it is conjugated. Modifying the alcohol with a reactive group such as chloride, tosylate, or mesylate allows Azido-dPEG®36-OH to function as a crosslinker through subsequent chemical manipulations.

As a click chemistry reagent, azido-dPEG®36-OH can participate in CuAAC, RuAAC, and SPAAC reactions. Because Vector Laboratories’ dPEG® products are amphiphilic, these reactions can occur almost equally well in water or organic solvents. The primary alcohol group on the opposite end of the linker can be used as a neutrally charged functional group. In this function, the alcohol enhances the water solubility of the conjugate molecule. Alternatively, the alcohol group can be functionalized with reactive groups and used to modify the conjugate further. Thus, azido-dPEG®36-OH can be used in conjugations with biomolecules where organic solvents may damage the conjugate target. This product was published in a 2014 paper demonstrating high-throughput modifications of siRNA via CuAAC.