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AZDye 647 Alkyne (CCT-1301)

This product was previously sold as 1126 from Fluoroprobes.

AZDye™ 647 Alkyne is a water-soluble, bright, far-red fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines and visualized with Cy5 filter sets. AZDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less azide detection (AZDye™ 647 DBCO or Cy5 DBCO) is also available for application where the presence of copper is not acceptable.

Description

AZDye™ 647 Alkyne is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines, and visualized with Cy5 filter sets.

AZDye™ 647 is often a reagent of choice for imaging of low abundance azide-containing biomolecules. AZDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used as a less expensive alternative to Alexa Fluor® 647 Azide.

For application where the presence of copper is not acceptable, please consider our AZDye™ 647 DBCO or Cy5 DBCO probes for copper-less detection of azide-modified molecules.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima648/671 nm
Extinction Coefficient
270,000
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar DyesAlexa Fluor® 647, CF® 647, DyLight® 649
Molecular weight896.07 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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