AZDye™ 488 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.
AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488.
DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific. Click Chemistry Tools is not affiliated with or endorsed by Thermo Fisher Scientific. AZDye™ 488 is NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Extinction Coefficient | 73,000 |
| Spectrally Similar Dyes | Alexa Fluor® 488, Atto™ 488, CF® 488 Dye, DyLight® 488, FITC |
| Molecular weight | 770.82 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF, MeOH |
| Purity | >95% (HPLC) |
| Appearance | Orange solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Dry ice |
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