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AZDye 532 DBCO (CCT-1282)

[text-backorder]

This product was previously sold as 1046 from Fluoroprobes.

Description

AZDye™ 532 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 532 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 532 DBCO is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

AZDye™ 532 is spectrally almost identical to many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima532/554 nm
Extinction Coefficient
78,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Spectrally Similar DyesAlexa Fluor® 532, Atto™ 532, CF® 532 dyes
Molecular weight872.92
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceOrange to light red solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Af Af

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