Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.

AZDye 647 Azide (CCT-1299)

[text-backorder]

This product was previously sold as 1124 from Fluoroprobes.

AZDye™ 647 Azide (formerly sold under AFDye™ 647 Azide label) is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe. Its excitation ideally suited for the 633 nm or 647 nm laser lines. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Description

AZDye™ 647 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye™ 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

AZDye™ 647 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used a less expensive alternative to Alexa Fluor® 647 Azide.

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Abs/Em Maxima 648/671 nm
Extinction Coefficient
270,000
Spectrally Similar Dyes Alexa Fluor® 647, CF® 647, DyLight® 649
Molecular weight 941.11 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Blue solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

Abs/Em Spectra

AF647

Selected References

  1. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci.135 (5), jcs258566. [PubMed]
  2. Lee, M.T., et al. (2020). Dietary sphinganine is selectively assimilated by members of themammaliangut microbiome. J Lipid Res . [PubMed]

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Log in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote