AZDye™ 647 Azide (formerly sold under AFDye™ 647 Azide label) is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe. Its excitation ideally suited for the 633 nm or 647 nm laser lines. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
AZDye™ 647 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye™ 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
AZDye™ 647 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used a less expensive alternative to Alexa Fluor® 647 Azide.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 648/671 nm |
| Extinction Coefficient |
270,000
|
| Spectrally Similar Dyes | Alexa Fluor® 647, CF® 647, DyLight® 649 |
| Molecular weight | 941.11 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Appearance | Blue solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
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