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AZDye 546 DBCO (CCT-1286)

[text-backorder]

This product was previously sold as 1057 from Fluoroprobes.

Description

AZDye™ 546 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice in live cell surface glycans and lipids studies where the presence of cytotoxic copper catalyst is not acceptable. In application where the presence of copper is a concern AZDye™ 546 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AZDye™ 546 dye is spectrally almost identical Alexa Fluor® 546, CF 543 and TAMRA.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima554/570 nm
Extinction Coefficient
110,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 nm or 546 nm
Spectrally Similar DyesAlexa Fluor® 546, Atto™ 546, CF® 546
Molecular weight1006.50
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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