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AZDye 405 Azide (CCT-1307)

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This product was previously sold as 1064 from Fluoroprobes.

AZDye™ 405 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, blue-fluorescent probe with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

Description

AZDye™ 405 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

AZDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima402/424 nm
Extinction Coefficient
35,000
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Spectrally Similar DyesAlexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
Molecular weight640.65 (protonated) 706.60 (sodium salt)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceYellow solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF405

Selected References

  1. Schuler, D., et al. (2021). Differential Labeling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis. Microorganisms9, 1578. [MDPI]

Applicable patents and legal notices are available at legal notices.

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