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AZDye 594 NHS Ester (FP-1101)

[text-backorder]

This product was previously sold as 1343 from Click Chemistry Tools.

Description

AZDye™ 594 NHS Ester is the most popular tool for modifying proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules with AZDye™ 594 label. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. AZDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima590/617 nm
Extinction coefficient92,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 594, CF™ 594, DyLight® 594
Molecular weight819.85
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF594

Protein Labeling Calculator

[protein-labeling-calculator]

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