AZDye 568 Maleimide (FP-1082)

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This product was previously sold as 1524 from Click Chemistry Tools.

Description

568Nm Laser

AZDye™ 568 Maleimide is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. AZDye™ 568 Maleimide is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima575/600 nm
Extinction coefficient92,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 568, CF™ 568
Molecular weight857.93
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Af568

Protein Labeling Calculator

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