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AZDye 555 NHS ester (FP-1166)

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This product was previously sold as 1341 from Click Chemistry Tools.

Description

AZDye™ 555 NHS Ester is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. Proteins can be labeled with AZDye 555 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AZDye™ 555 conjugates ideally suited for detection of low abundance targets.

The NHS Ester reacts specifically and efficiently with a primary amine at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima555/572 nm
Extinction coefficient155,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesCy3, DyLight® 550, Alexa Fluor® 555
Molecular weight930.04 (protonated)
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF555

Protein Labeling Calculator

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