Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.
AZDye™ 546 Tyramide (Alexa Fluor® 546 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).
| Unit Size | 150 slides, 1500 slides |
|---|---|
| Abs/Em Maxima | 543/563 nm |
| Extinction Coefficient | 105,000 |
| Flow Cytometry Laser Line | 532 nm |
| Microscopy Laser Line | 543 or 546 nm |
| Spectrally Similar Dyes | Alexa Fluor® 546, Atto™ 543, CF® 543 |
| Molecular weight | 939.08 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF, MeOH |
| Appearance | Red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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