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AZDye 546 Tyramide (CCT-1539)

Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 546 Tyramide (Alexa Fluor® 546 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima543/563 nm
Extinction Coefficient
105,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 or 546 nm
Spectrally Similar DyesAlexa Fluor® 546, Atto™ 543, CF® 543
Molecular weight939.08
CASN/A
SolubilityWater, DMSO, DMF, MeOH
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Mb Mb Vectorlabs

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