AZDye™ 546 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
AZDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||554/570 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
543 nm or 546 nm
|Spectrally Similar Dyes||Alexa Fluor® 546, Atto™ 546, CF® 546|
|Molecular weight||1050.39 (protonated)|
|Solubility||Water, DMSO, DMF|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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