AZDye™ 532 NHS Ester is an amine reactive, yellow-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.
AZDye™ 532 is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Abs/Em Maxima||530/555 nm|
|Extinction coefficient||81,000 cm-1M-1|
|Solubility||Water, DMSO, DMF|
|Spectrally similar dyes||Alexa Fluor® 532, DyLight® 532|
|Shipping Conditions||Ambient temperature|
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