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AZDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AZDye 488 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.
AZDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye™ 488 dyes are best suited to direct imaging of low-abundance targets.
| Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
|---|---|
| Reactivity | Aldehyde, ketone |
| Abs/Em Maxima | 494/517 nm |
| Extinction coefficient | 73,000 cm-1M-1 |
| Solubility | Water, DMSO, DMF |
| Spectrally similar dyes | Alexa Fluor® 488, CF™ 488 |
| Molecular weight | 606.58 (protonated) |
| Storage Conditions | -20°C. |
| Shipping Conditions | Ambient temperature |
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