AZDye™ 488 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AZDye™ 488 Biotin is a water soluble reagent and its green fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.
AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A.
|Unit Size||1 mg, 5 mg|
|Abs/Em Maxima||494/517 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
|Spectrally Similar Dyes||FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488|
|Molecular weight||935.00 (protonated)|
|Solubility||Water, DMSO, DMF|
|Appearance||Orange to light red solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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