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AZDye 350 NHS Ester (FP-1002)

This product was previously sold as 1336 from Click Chemistry Tools.

Description

VectorlabsAZDye™ 350 NHS Ester (Alexa Fluor® 350 NHS Ester equivalent) is an amine reactive, water-soluble, blue-emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

AZDye™ 350 NHS Ester is structurally identical to Alexa Fluor® 350 NHS Ester.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amines
Abs/Em Maxima346/445 nm
Extinction coefficient19,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 350, AMCA, DyLight® 350
Molecular weight410.35
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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