AZDye™ 350 Acid is an amine reactive, non-activated, water-soluble, blue-emitting dye used for derivatizing amine-containing molecules in the presence of activators (e.g. EDC or HATU) through a stable, covalent amide bond. The NHS ester (or succinimidyl ester) of this dye is also available for conjugating AZDye™ 350 to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.
AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for producing bright, blue fluorescent conjugates for imaging and flow cytometry. The emission of AZDye™ 350 conjugates is slightly shorter than that of AMCA or AMCA conjugates, reducing AZDye™ 350’s spectral overlap with the emission of fluorescein or Alexa Fluor® 488 dye.
AZDye™ 350 dye structurally is identical to Alexa Fluor® Dye 350.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Reactivity||Primary amines (needs activation)|
|Abs/Em Maxima||346/445 nm|
|Extinction coefficient||19,000 cm-1M-1|
|Solubility||Water, DMSO, DMF|
|Spectrally similar dyes||Alexa Fluor® 350, AMCA, DyLight® 350|
|Shipping Conditions||Ambient temperature|
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