AZDye™ 350 Acid is an amine reactive, non-activated, water-soluble, blue-emitting dye used for derivatizing amine-containing molecules in the presence of activators (e.g. EDC or HATU) through a stable, covalent amide bond. The NHS ester (or succinimidyl ester) of this dye is also available for conjugating AZDye™ 350 to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.
AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for producing bright, blue fluorescent conjugates for imaging and flow cytometry. The emission of AZDye™ 350 conjugates is slightly shorter than that of AMCA or AMCA conjugates, reducing AZDye™ 350’s spectral overlap with the emission of fluorescein or Alexa Fluor® 488 dye.
AZDye™ 350 dye structurally is identical to Alexa Fluor® Dye 350.
Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
---|---|
Reactivity | Primary amines (needs activation) |
Abs/Em Maxima | 346/445 nm |
Extinction coefficient | 19,000 cm-1M-1 |
Solubility | Water, DMSO, DMF |
Spectrally similar dyes | Alexa Fluor® 350, AMCA, DyLight® 350 |
Molecular weight | 313.27 |
Storage Conditions | -20°C |
Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
Stay in the Loop. Join Our Online Community
Products
Ordering
About Us
Application
Resources
©Vector Laboratories, Inc. 2025 All Rights Reserved.
To provide the best experiences, we use technologies like cookies to store and/or access device information. Consenting to these technologies will allow us to process data such as browsing behavior or unique IDs on this site. Not consenting or withdrawing consent, may adversely affect certain features and functions. Privacy Statement
How do I Request a Quote?
To request a quote for products: