AZDye 350 Acid (FP-1003)


AZDye™ 350 Acid is an amine reactive, non-activated, water-soluble, blue-emitting dye used for derivatizing amine-containing molecules in the presence of activators (e.g. EDC or HATU) through a stable, covalent amide bond. The NHS ester (or succinimidyl ester) of this dye is also available for conjugating AZDye™ 350 to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for producing bright, blue fluorescent conjugates for imaging and flow cytometry. The emission of AZDye™ 350 conjugates is slightly shorter than that of AMCA or AMCA conjugates, reducing AZDye™ 350’s spectral overlap with the emission of fluorescein or Alexa Fluor® 488 dye.

AZDye™ 350 dye structurally is identical to Alexa Fluor® Dye 350.


Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amines (needs activation)
Abs/Em Maxima346/445 nm
Extinction coefficient19,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 350, AMCA, DyLight® 350
Molecular weight313.27
Storage Conditions-20°C
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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