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AZDye™ 350 Acid is an amine reactive, non-activated, water-soluble, blue-emitting dye used for derivatizing amine-containing molecules in the presence of activators (e.g. EDC or HATU) through a stable, covalent amide bond. The NHS ester (or succinimidyl ester) of this dye is also available for conjugating AZDye™ 350 to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.
AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for producing bright, blue fluorescent conjugates for imaging and flow cytometry. The emission of AZDye™ 350 conjugates is slightly shorter than that of AMCA or AMCA conjugates, reducing AZDye™ 350’s spectral overlap with the emission of fluorescein or Alexa Fluor® 488 dye.
AZDye™ 350 dye structurally is identical to Alexa Fluor® Dye 350.
| Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
|---|---|
| Reactivity | Primary amines (needs activation) |
| Abs/Em Maxima | 346/445 nm |
| Extinction coefficient | 19,000 cm-1M-1 |
| Solubility | Water, DMSO, DMF |
| Spectrally similar dyes | Alexa Fluor® 350, AMCA, DyLight® 350 |
| Molecular weight | 313.27 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient temperature |
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