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Streptavidin, DyLight™ 649

SA-5649-1
SKU Unit Size Price Qty
SA-5649-1 1 mg
$187.00
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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Excitation at 655 nm
  • Emission at 670 nm
  • Color:  Far Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 655 nm
Maximum Emission 670 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 649
Color of Fluorescence Far Red
Format Concentrate

Documents

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3D STORM imaging of Alexa Fluor 647 stained microtubules in a VECTASHIELD Mounting Medium/TRIS-Glycerol mixture. (Image kindly supplied by Dr. Nicolas Olivier, King's College, London UK.) VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10
Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd. VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10
FastTag Fluorescein labeled pUC1.77 detected with Biotinylated Anti-Fluorescein and Fluorescein Avidin DCS, and counterstained with VECTASHIELD with PI. VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
Prostate: Prostate Specific Antigen (rp), fluorescein labeled using the ProtOn Fluorescein Labeling Kit. Propidium Iodide counterstain (red). Mounted in VECTASHIELD HardSet Mounting Medium. VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.* VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse anti-mouse IgG and mounted in VECTASHIELD HardSet Mounting Medium with DAPI and TRITC-Phalloidin. VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10

Citations

Technical Information

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

*Additional information about the images can be found here.

 

DyLight Spectra

DyLight Table

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