Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | Recommended concentration range for use5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Excitation | 655 nm |
| Maximum Emission | 670 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 1.0 mg/ml active conjugate |
| Conjugate | DyLight 649 |
| Color of Fluorescence | Far Red |
| Format | Concentrate |
Streptavidin/Biotin Blocking Kit
Catalog #:
SP-2002
Goat Anti-Streptavidin Antibody, Biotinylated
Catalog #:
BA-0500-.5
VECTASHIELD® Antifade Mounting Medium
Catalog #:
H-1000-10
VECTASHIELD® Antifade Mounting Medium with DAPI
Catalog #:
H-1200-10
VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI)
Catalog #:
H-1300-10
VECTASHIELD® HardSet™ Antifade Mounting Medium
Catalog #:
H-1400-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI
Catalog #:
H-1500-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin
Catalog #:
H-1600-10
Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.
DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.
*Additional information about the images can be found here.
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