Ready-to-Use (R.T.U.) Biotinylated Goat Anti-Rabbit IgG Secondary Antibody is a stabilized, prediluted, ready-to-use solution of our biotinylated secondary antibodies. It is used in an avidin-biotin or streptavidin-biotin detection system.
Features:
Recognizes both heavy and light chains (H+L)
Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
Ready-to-use solution of biotinylated goat anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage
2-8 °C
Recommended Usage
Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in
buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®® ABC Reagent (PK-7100).
* 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.