Format Ready-to-Use | |
Unit Size 50 ml | |
Storage Instructions 2-8 °C | |
Usage Summary Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs | |
Target Species Mouse | |
Concentration Ready-to-use solution of biotinylated goat anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative. | |
Conjugate Biotinylated | |
Reactive Species Goat | |
Source Species Mouse | |
Host Species Goat |
Goat Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U.
Features::
• Affinity-purified, ultrapure, high affinity antibody
• Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
• Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
• Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
• Can be used for tissue and cell staining, ELISAs, and blots
• Supplied in a convenient ready-to-use prediluted, stabilized solution
$187.00
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Technical Information Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots. Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use. Biotinylated Goat Anti-Mouse IgG (H+L) is supplied in solution. With some exceptions the recommended dilution for most applications is 1:200. (H+L) indicates the antibody recognizes both heavy and light chains. |
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Product FAQs
I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN® Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?
Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.
