Nucleic acid probes are frequently labeled with digoxigenin (DIG), a steroid found in plants. This is particularly common in double-label in situ hybridization applications with a biotin-labeled probe. DIG-labeled probes can be visualized using our high affinity, purified antibody against digoxigenin/digoxin.
|Unit Size||1 mg|
|Applications||In situ hybridization, Blotting Applications|
|Concentration||1 mg active IgG/ml|
|Recommended Storage||2-8 °C|
|Solution||10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.|
|Recommended Usage||This product is recommended for use in transfer blot applications (Northern, Southern, Western, dot blots, etc.) and in situ hybridization. The recommended concentration range for use is 0.5-5 µg/ml.This reagent can be detected using a number of anti-goat IgG secondary antibody options.|
|Detection Target||Digoxigenin/Digoxin (DIG)|
Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for applications such as in situ hybridization. DIG-labeled probes are detected with antibodies specifically directed against the DIG label. We have generated high affinity and highly purified antibodies for this purpose. The unconjugated anti-DIG antibody can be used to detect DIG-labeled probes employing labeled anti-goat antibodies or our ImmPRESS® Anti-Goat detection systems.
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