Cy7 Alkyne

The Cy7 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Cy7 Azide is a bright and photostable near-IR probe that is spectrally similar to Alexa Fluor® 750, DyLight® 750, and IRDye® 750 dye. The fluorescence of Cy7 Azide is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.