5-Carboxy-X-rhodamine N-succinimidyl ester (5-ROX NHS Ester) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to “direct-load” DNA sequencing. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.
| Unit Size | 25 mg, 100 mg, 1000 mg |
|---|---|
| Reactivity | Primary amines |
| Abs/Em Maxima | 582/606 nm |
| Extinction coefficient | 93,000 cm-1M-1 |
| Solubility | DMSO, DMF, MeOH |
| Spectrally similar dyes | Alexa Fluor® 568, TAMRA, CF™ 568 |
| CAS | 209734-74-7 |
| Molecular weight | 631.69 |
| Storage Conditions | -20°C. |
| Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
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