5-ROX (5-Carboxy-X-Rhodamine) (FP-1271)


The carboxylic acid of 5-ROX is used for oligonucleotide labeling and automated DNA sequencing applications. Often a dye of choice for fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.


Unit Size25 mg, 100 mg, 1000 mg
ReactivityPrimary amines
Abs/Em Maxima570/591 nm
Extinction coefficient93,000 cm-1M-1
SolubilityDMSO, DMF, MeOH
Spectrally similar dyesAlexa Fluor® 568, TAMRA, CF™ 568
Molecular weight534.61
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Protein Labeling Calculator


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