The carboxylic acid of 5-ROX is used for oligonucleotide labeling and automated DNA sequencing applications. Often a dye of choice for fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.
|Unit Size||25 mg, 100 mg, 1000 mg|
|Abs/Em Maxima||570/591 nm|
|Extinction coefficient||93,000 cm-1M-1|
|Solubility||DMSO, DMF, MeOH|
|Spectrally similar dyes||Alexa Fluor® 568, TAMRA, CF™ 568|
|Shipping Conditions||Ambient temperature|
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