Alkaline Phosphatase (AP) Substrates

Vector offers alkaline phosphatase (AP) substrates in both original and ImmPACT™ formulations.

The original Vector® AP substrates offer a range of color choices and the following advantages:

  • Allow you to stain antigens in pigmented tissues and melanomas
  • Enable localization of multiple antigens in a single tissue section (the combination of Vector® Red and Vector® Blue substrate, for example, are useful in co-localization experiments)
  • Vector® Red and Vector® Blue substrates can be viewed with both conventional light microscopy as well as fluorescence miscroscopy
  • Vector® AP substrates can be both non-aqueous and aqueously mounted with the exception of Vector® Black, which should only be non-aqueously mounted
  • Vector® Blue substrate generates a product that can be cleared with citrus oil-based clearing agents and then permanently mounted
  • The BCIP/NBT substrate is frequently used for nitrocellulose or in situ hybridization applications

Our newer ImmPACT™ Vector® Red Substrate is the most sensitive red AP substrate:

  • 2-4 fold greater sensitivity than the original substrate
  • Supplied with stock solutions in dropper bottles and optimized diluent
  • Optimized specifically for immunohistochemistry applications
  • Can be viewed with fluorescence as well as light microscopy

Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.

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IHC Multiplexing Guide

A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures. A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.

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Enzyme Substrates Brochure

In addition to photomicrographs which help the user visualize the color that is generally produced by each substrate, this brochure contains information on substrate properties especially useful for those who wish to multiplex (or introduce multiple labels in the same tissue section):  table of substrate properties, a schematic of relative senstitivities of substrate formulations, and a table of pairwise substrate compatibility.

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