Vector Laboratories’ blog provides a wealth of information on immunohistochemistry (IHC), immunofluorescence (IF), and related life science research topics.
Hypersialylation of glycans is one of the key drivers in cell differentiation in cancer, including pancreatic ductal adenocarcinoma (PDAC) cells. In this article, we review a study investigating how this mechanism led to the differentiation... Read more
In this publication highlight, we review the use of LIBRA-seq to characterize monoclonal antibodies from a recovered SARS-CoV donor, expanding our understanding of cross-reactive antibodies for coronavirus.
Eric Peterson has long held an interest in the underpinnings of life, found enjoyment in science fiction, and taken a practical approach to career choices rooted in economics. He is not the guy who plotted... Read more
When scientists detect targets of interest with immunohistochemistry (IHC), antibody binding and substrate detection alone often produce a perplexing picture where the target lacks context. Luckily there’s a solution. Counterstaining IHC samples after enzymatic detection... Read more
In this publication highlight, we review a study examining the impact of macrophage-derived microparticles on the tumor microenvironment and their synergistic effect on anti-PD-1 antibody activity.
When it comes to selecting the substrate for your IHC application, you have variety and flexibility, and our focus is to help you make the right decision. This blog discusses different factors to consider—enzyme, sensitivity,... Read more
When performing IHC, scientists aspire to reduce background or non-specific staining on the specimen. Explore how you can block the sources of non-specific signals increases the signal-to-noise ratio for optimal results.
When Courtney Young was a senior in high school, her 15 year old cousin was diagnosed with Duchenne muscular dystrophy (DMD). This diagnosis provided her with a strong motivation to pursue a future in science,... Read more
Bioconjugation offers a way to chemically link two molecules to form a single hybrid, where at least one of the molecules in the partnership is a biomolecule. The resulting product retains the activity of each... Read more
When everything goes right, looking through the microscope at your immunofluorescence-stained tissue is no less beautiful than peering through a telescope at the night sky. Unfortunately, experimental issues can obscure that pristine view of your... Read more
