S-acetyl-dPEG®₄-OH

S-acetyl-dPEG®4-OH, product number QBD-10156, contains an acetyl-protected thiol on a short, hydroxy-terminated, discrete polyethylene glycol (dPEG®) linker. This product is designed for the thiolation of biomolecules.

Bioconjugation frequently employs thiolation because the reactions to install thiol groups on molecules or to react with molecules containing sulfhydryl groups are relatively simple and often chemoselective. The thioacetyl group is intended to install an acetyl-protected thiol onto biomolecules. While installing a protected thiol through a reaction between a carboxylic acid and a primary amine is widely popular in bioconjugation technology, it is not always possible or feasible. S-acetyl-dPEG®4-OH permits the installation of a protected thiol through a short, hydroxy-terminated discrete PEG® linker that can be installed by the reaction of hydroxy group with an activated carboxylic acid ester or isothiocyanate group. Hydroxylamine hydrochloride (CAS number 5470-11-1) easily removes the acetyl group that protects the thiol, thus exposing the sulfhydryl for reaction. One application for S-acetyl-dPEG®4-OH is the modification of nucleic acids using phosphoramidite chemistry.