Format Concentrate | |
Formulation 10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative | |
Unit Size 1 mg | |
Storage Instructions 2-8 °C, DO NOT FREEZE | |
Usage Summary Important Note: The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present. If this antibody is to be used in thse applications, blocking with Animal-Free blocker™ solution (Cat. No. SP-5030) is recommended. Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20. Working concentration of HRP-anti goat IgG is 0.5-5.0 µg/ml. | |
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs | |
Target Species Goat | |
Concentration 1 mg active conjugate/ml | |
Conjugate Peroxidase | |
Safety Title WARNING: Cancer –www.P65Warnings.ca.gov | |
Reactive Species Horse | |
Source Species Goat | |
Host Species Horse |
Horse Anti-Goat IgG Antibody (H+L), Peroxidase
Features:
• Affinity-purified, ultrapure, high affinity antibody
• Suitable for use with goat, sheep, and bovine IgG primary antibodies
• Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
• Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
• Cross-linked with the highest specific activity horseradish peroxidase
• Supplied in solution
$190.00
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Technical Information Our high affinity Horse Anti-Goat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots. As well as recognizing goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies. The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended. |
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