MB 660R Alkyne is often a reagent of choice for imaging of low abundance azide-containing biomolecules. AZDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
MB 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. MB 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. MB 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of MB 660R make the dye an ideal choice for confocal microscopy and other demanding applications.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||665/690 nm|
|Flow Cytometry Laser Line|
633 or 635 nm
|Microscopy Laser Line|
633 or 635 nm
|Spectrally Similar Dyes||Alexa Fluor® 660, CF® 660R|
|Molecular weight||781.93 (protonated)|
|Solubility||Water, DMSO, DMF|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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