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L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.
| Unit Size | 25 mg, 100 mg, 1000 mg, 5 g |
|---|---|
| Molecular weight | 180.59 (HCl salt) |
| Chemical composition | C4H9ClN4O2 (HCl salt) |
| CAS | 942518-29-8 |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (H NMR) |
| Appearance | White crystalline |
| Storage Conditions | -20°C. |
| Shipping Conditions | Ambient temperature |
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