IR 650 DBCO (IRDye® 650 DBCO analog) is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
IR 650 DBCO is a bright, far-red-fluorescent, pH insensitive azide-reactive probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. IR 650 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern IR 650 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Reactivity||Azide, picolyl azide|
|Abs/Em Maxima||651/668 nm|
|Extinction coefficient||230,000 cm-1M-1|
|Solubility||Water, DMSO, DMF|
|Spectrally similar dyes||Cy5, Alexa Fluor® 647, DyLight® 649,|
|Shipping Conditions||Ambient temperature|
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