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Click Chemistry Tools manufactures Streptavidin Magnetic Beads with one of the highest biotin binding capacities on the market (>12 nmol of biotin per mg). Higher binding translates into reduced bead mass required to immobilize a biotinylated sample and lower background noise from nonspecific binding, resulting in better signals and lower net costs. The beads are 1.0 µm in diameter and bind at least 12 nmol of free biotin per milligram of beads. The beads also have excellent magnetic properties for rapid and efficient capture using a variety of magnetic stands.
Binding capacity: ≥ 12 nmol/mg of free biotin per mg of beads and ≥ 110 µg/mg of biotinylated IgG per mg of beads.
Excellent for fast and direct isolation of any biotinylated molecules, such as nucleic acids (both DNA and RNA), oligonucleotides, proteins, peptides, and antibodies.
Easily adapted for manual and automated high-throughput applications.
High batch-to-batch reproducibility.
30 µg of BSA-biotin in HEK 293T RIPA lysate was mixed with 1 mg of High Capacity Streptavidin Magnetic Beads. Eluted with 1x Laemmli Sample Buffer, 10 minutes at 95 C. Mass shift – conjugate of BSA-biotin and one subunit of Streptavidin.
| Unit Size | 1 ml, 5 ml |
|---|---|
| Enrichment target | biotinylated molecules |
| Activation level (free biotin per milligram of beads) | ≥ 12 nmol /mg |
| Activation level (biotinylated IgG per milligram of beads) | ≥ 110 µg/mg |
| Support | Highly uniformed supermagnetic particles |
| Coating | Highly crosslinked, proprietary (non-styrene) polymer matrix |
| Mean bead diameter | 0.8 µm |
| Formulation | 10 mg/mL suspension in water |
| Preservative | 0.05% sodium azide |
| Appearance | Brown suspension |
| Storage | 2-8C |
| Shipping Conditions | Ambient temperature |
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