Format Concentrate | |
Formulation 10 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 0.05% thimerosal | |
Unit Size 1 mg | |
Storage Instructions 2-8 °C, DO NOT FREEZE | |
Usage Summary Moles peroxidase/mole IgG:3.3 Recommended diluent for microtitration plate assays: 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP anti-biotin is 0.5 – 5.0 µg/ml. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs | |
Concentration 1 mg active conjugate/ml | |
Conjugate Peroxidase | |
Safety Title WARNING: Cancer –www.P65Warnings.ca.gov | |
Reactive Species Goat | |
Host Species Goat |
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This product has been discontinued.
Please consider the following as potential substitutes: SP-3000-1. Goat Anti-Biotin, Unconjugated. Or contact Technical Support @ [email protected] for assistance in finding alternative.
Goat Anti-Biotin
Peroxidase conjugated anti-biotin antibodies are produced with the highest specific acitivity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody is chosen to provide stable, highly active conjugates for immunohistochemical, in situ hybridization, enzyme immunoassay, or transfer blot applications.
$172.00
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Technical Information Our high affinity polyclonal anti-biotin antibody is produced by hyperimmunizing goats with biotin and is purified by affinity chromatography. Anti-Biotin is available unconjugated, bound to heat stable, cross-linked agarose beads or labeled with enzymes or fluorescein. Agarose bound Anti-Biotin contains 1 mg of Anti-Biotin per ml of settled 4% agarose gel. This product can be used to bind and dissociate biotinylated molecules. Fluorescein labeled Anti-Biotin has been optimally conjugated to provide a bright fluorescent signal. The horseradish peroxidase and alkaline phosphatase conjugates have been produced with the highest specific activity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody was chosen to provide stable, highly active conjugates for immunohistochemical, enzyme immunoassay, or transfer blot applications. |
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