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Goat Anti-Biotin, Peroxidase Conjugated (SP-3010-1)

Purchase this product from 2BScientific

Description

Peroxidase conjugated anti-biotin antibodies are produced with the highest specific acitivity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody is chosen to provide stable, highly active conjugates for immunohistochemical, in situ hybridization, enzyme immunoassay, or transfer blot applications.

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration1 mg active conjugate/ml
Recommended Storage2-8 °C, DO NOT FREEZE
Solution10 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 0.05% thimerosal
Recommended UsageMoles peroxidase/mole IgG:3.3 Recommended diluent for microtitration plate assays: 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP anti-biotin is 0.5 - 5.0 µg/ml. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase.
Detection TargetBiotin
ConjugatePeroxidase
Host SpeciesGoat
FormatConcentrate
Safety TitleWARNING: Cancer - www.P65Warnings.ca.gov

Citations

Technical Information

Our high affinity polyclonal anti-biotin antibody is produced by hyperimmunizing goats with biotin and is purified by affinity chromatography. Anti-Biotin is available unconjugated, bound to heat stable, cross-linked agarose beads or labeled with enzymes or fluorescein. Agarose bound Anti-Biotin contains 1 mg of Anti-Biotin per ml of settled 4% agarose gel. This product can be used to bind and dissociate biotinylated molecules. Fluorescein labeled Anti-Biotin has been optimally conjugated to provide a bright fluorescent signal. The horseradish peroxidase and alkaline phosphatase conjugates have been produced with the highest specific activity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody was chosen to provide stable, highly active conjugates for immunohistochemical, enzyme immunoassay, or transfer blot applications.

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