Molecular Weight 36 | |
Extinction Coefficient 1.46 | |
Inhibiting or Eluting Sugar GlcNAc or Chitin Hydrolysate | |
Unit Size 10 mg, 25 mg | |
Storage Instructions 2-8 °C | |
Sugar Specificity Terminal GlcNAc-β, terminal GlcNAc-a and terminal N-acetyl-containing glycans | |
Usage Summary Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. | |
Applications Immunohistochemistry, Immunofluorescence | |
Conjugate Unconjugated |
Wheat Germ Agglutinin (WGA), Unconjugated
The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).
Price range: $186.00 through $326.00
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Technical Information Wheat germ agglutinin (WGA) contains a group of closely related isolectins, with an isoelectric point about pH 9. This lectin is used for the purification of insulin receptors and for neuronal tracing. Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required |
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