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Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Biotinylated

B-1205-.5
SKU Unit Size Price Qty
B-1205-.5 500 ug
$175.00
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Description

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.  This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. 

Biotinylated GSL I-B4 has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

More Information
Unit Size 500 ug
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 0.5 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Galactose

Documents

Product FAQs

I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

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Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Fluorescein Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Fluorescein Catalog #: FL-1201-.5
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
Mouse tongue (formalin-fixed, paraffin-embedded) stained with DyLight 594-Labeled GSL 1, Isolectin B4 (red) and counterstained with DAPI (blue). Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, DyLight™ 594 Catalog #: DL-1207-.5

Citations

Technical Information

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting Sugar: 500 mM galactose or 100 mM raffinose
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