Molecular Weight 111 | |
Color of Fluorescence Green | |
Extinction Coefficient 1.22 | |
Formulation 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 | |
Inhibiting or Eluting Sugar GalNAc | |
Maximum Emission 514-521 nm | |
Maximum Excitation 495-500 nm | |
Unit Size 2 mg, 5 mg | |
Storage Instructions 2-8 °C | |
Sugar Specificity Forssman antigen and GM2 | |
Usage Summary The recommended concentration range for use is 5-20 µg/ml. | |
Applications Immunofluorescence | |
Concentration FL-1031-2 (2 mg active conjugate/ml)FL-1031-5 (5 mg active conjugate/ml) | |
Conjugate Fluorescein |
Dolichos Biflorus Agglutinin (DBA), Fluorescein
Dolichos biflorus agglutinin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
Fluorescein labeled Dolichos biflorus agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Price range: $195.00 through $319.00
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Technical Information Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine |
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