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Dolichos Biflorus Agglutinin (DBA), Fluorescein (FL-1031)

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Description

Dolichos biflorus agglutinin has a carbohydrate specificity toward α-linked N-acetylgalactosamine.  It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.

Fluorescein labeled Dolichos biflorus agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size2 mg, 5 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
ConcentrationFL-1031-2 (2 mg active conjugate/ml)
FL-1031-5 (5 mg active conjugate/ml)
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityN-Acetylgalactosamine

Citations

Technical Information

Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size. 

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

Applicable patents and legal notices are available at legal notices.

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