Yes, the same ImmPRESS peroxidase secondary detection reagent can be used for double staining. There are a few considerations. First, we would suggest optimizing each single stain first on different tissue sections. Once the conditions for each stain have been established, the single assays should be run sequentially, from primary antibody though substrate color development, to achieve double staining on the same section. Secondly, different peroxidase substrates will have to be used to provide adequate color contrast and reliable target antigen localization. This double staining approach is most reproducible when detecting antigens expressed in different cell types on the same section, or different cell compartments of the same cell type.
Please see our multiple antigen labeling guide for additional considerations: