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ImmPRESS® Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta)

MP-7714
SKU Unit Size Price Qty
MP-7714-15 1 kit
$602.00
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Description

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-rabbit IgG and anti-mouse IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High Sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Rabbit IgG and ImmPRESS  AP Anti-Mouse IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

 

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry
Technology Micropolymer Reagents
Detection Enzyme(s) HRP/AP
Conjugate Micropolymer HRP/Micropolymer AP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use
Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

Documents

Related Products

Tonsil (paraffin): ImmPRESS Duet Kit (MP-7724) used to detect mouse anti- CD34 (ImmPACT DAB, brown) and rabbit anti-Ki67 (ImmPACT Vector® Red, magenta) antibodies. ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta) Catalog #: MP-7724
Dog intestine stained with mouse antibody against multi-cytokeratin and detected with ImmPRESS VR HRP Anti-Mouse IgG and Vector NovaRED Substrate. Counterstained with Vector Hematoxylin QS. ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent Catalog #: MP-6402-15
Prostate: PSA (m), VECTASTAIN ABC-AP Mouse IgG Kit, BCIP/NBT (indigo), viewed under bright field microscopy. BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) Catalog #: SK-5400
Tonsil: Cytokeratin (AE1/AE3 (m), ImmPRESS-AP Reagent, Vector Blue AP Substrate (blue). Vector® Blue Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5300
Prostate: Anti-PSA (mouse monoclonal, VP-P981), ImmPRESS™ AP Anti-Mouse Ig Reagent (MP-5402), ImmPACT™ Vector® Red Alkaline Phosphatase Substrate (SK-5105), viewed under brightfield microscopy. ImmPACT® Vector® Red Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5105
Human tonsil (FFPE): CD45 (rat) detected with ImmPRESS Goat Anti Rat IgG and ImmPACT VIP (purple). No Counterstain. ImmPRESS® HRP Goat Anti-Rat IgG Polymer Detection Kit, Peroxidase Catalog #: MP-7404-50
Rat Intestine: Desmin (m), ImmPRESS Anti-Mouse Ig Kit (Rat Adsorbed), DAB (brown) Substrate Kit. Hematoxylin QS (blue) counterstain. ImmPRESS® HRP Horse Anti-Mouse IgG, Rat adsorbed Polymer Detection Kit, Peroxidase Catalog #: MP-7422-15
Human Tonsil (FFPE): CD45 (rat) detected with ImmPRESS Goat Anti-Rat IgG (Mouse Adsorbed) and ImmPACT AEC Substrate (red). Counterstained with Vector Hematoxylin QS (blue). ImmPRESS® HRP Goat Anti-Rat IgG, Mouse adsorbed Polymer Detection Kit, Peroxidase Catalog #: MP-7444-15
Normal Horse Serum Blocking Solution, 2.5% Normal Horse Serum Blocking Solution, 2.5% Catalog #: S-2012-50
Prostate: Prostate Specific Antigen (m), ImmPRESS Reagent (HRP), ImmPACT DAB (brown). ImmPACT® DAB Substrate Kit, Peroxidase (HRP) Catalog #: SK-4105
Tonsil: CD20 (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT VIP (purple) substrate. ImmPACT® VIP Substrate Kit, Peroxidase (HRP) Catalog #: SK-4605
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT SG (blue-gray). ImmPACT® SG Substrate Kit, Peroxidase (HRP) Catalog #: SK-4705
Tonsil: Cytokeratin (AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT NovaRED Substrate (red). ImmPACT NovaRED® Substrate Kit, Peroxidase (HRP) Catalog #: SK-4805
ImmPACT® AEC Substrate Kit, Peroxidase (HRP) ImmPACT® AEC Substrate Kit, Peroxidase (HRP) Catalog #: SK-4205

Technical Information

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.  These reagents are blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT DAB EqV (HRP, brown) and ImmPACT Vector® Red (AP, magenta) substrates are included to complete this easy to use staining kit.

Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

Also included, BLOXALL  Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.

All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.

 

Product review on Biocompare.com.

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