The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

 

DyLight^™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. 

DyLight^™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight^™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight^™ dyes can be applied as single labels or in combination with other DyLight^™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight^™ dyes currently offered are DyLight^™ 488 (green), DyLight^™ 549 (orange), DyLight^™ 594 (red), and DyLight^™ 649 (far red).