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Disulfide Azide Agarose is a 6% crosslinked agarose resin that is activated with azide groups through a cleavable disulfide linker. Once biomolecules are covalently captures to the resin, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins. Cleavage under reducing conditions, such as 2% 2-Mercaptoethanol, yields a highly enriched population of proteins. This product is adequate to work in batch or column purification (low pressure).
| Unit Size | 2 ml |
|---|---|
| Enrichment target | Alkyne-modified proteins |
| Format | Batch or column purifications |
| Activation level | 5-20 µmol azide groups per mL resin |
| Bead size | Spherical, 50-150 µm |
| Formulation | 50% slurry |
| Molecular weight left behind | 188.28 |
| Shipping Conditions | Ambient temperature |
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