Disulfide Azide Agarose is a 6% crosslinked agarose resin that is activated with azide groups through a cleavable disulfide linker. Once biomolecules are covalently captures to the resin, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins. Cleavage under reducing conditions, such as 2% 2-Mercaptoethanol, yields a highly enriched population of proteins. This product is adequate to work in batch or column purification (low pressure).
|Unit Size||2 ml|
|Enrichment target||Alkyne-modified proteins|
|Format||Batch or column purifications|
|Activation level||5-20 µmol azide groups per mL resin|
|Bead size||Spherical, 50-150 µm|
|Molecular weight left behind||188.28|
|Shipping Conditions||Ambient temperature|
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