Cy5 DBCO

Cy5 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Price range: $153.00 through $3,223.00

Category: Cy5 Dyes

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SKU: CCT-A130

CAS Number 1564286-24-3
Molecular Weight 1211.6
Molecular Formula C64H86N6O11S3
Appearance Blue solid
Chemical Formula C64H86N6O11S3
Extinction Coefficient 250,000
Purity >95% (HPLC)
Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Solubility Water, DMSO, DMF
Storage Instructions -20°C. Desiccate
Spectrally Similar Dyes Alexa Fluor® 647, CF™ 647 Dye, DyLight™649
Laser Line 633 or 635 nm
Excitation/Emission Maximum 649 nm / 666 nm
Shipping Conditions Ambient temperature
Shipping Instructions Ambient temperature

Abs/Em Spectra

Cy5

Selected References

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Song, S., et al. (2020). In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjugate Chem., 31(5), 1562-74. [PubMed]
Valentini, T.V., et al. (2020). Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota. Nat Commun., 11, 2287-74. [PMC]
Zhang, C., et al. (2016). In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy. Proc Natl Acad Sci U S A., 113 (19), 5173-8. [PubMed]
Kim, S. H., et al. (2014). Cell labeling and tracking method without distorted signals by phagocytosis of macrophages . Theranostics, 4 (4), 420-31. [PubMed]