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Sambucus Nigra Lectin (SNA, EBL), CY3 (CL-1303-1)

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Description

Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis.

Cy3 labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. 

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation552 nm
Maximum Emission565 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.
Concentration1 mg active conjugate/ml
ConjugateCy3
Color of FluorescenceRed
Sugar SpecificitySialic Acid

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. 

Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid

Applicable patents and legal notices are available at legal notices.

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