Click-&-Go® Protein Enrichment Kit is an efficient tool for capture of azide-modified proteins on an alkyne-agarose resin. The azido-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the alkyne-agarose resin supplied. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon protease digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.
|Unit Size||1 Kit|
|Enrichment target||Azide-modified proteins|
|Isolation technology||Biotin-free covalent capture|
|Shipping Conditions||Ambient temperature|
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