Format Concentrate | |
Formulation 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. | |
Unit Size 1.5 mg | |
Storage Instructions 2-8 °C; Store frozen for long term storage | |
Usage Summary The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note: The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. Note:This product has the same concentration as that provided in the VECTASTAIN® ABC kits. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs | |
Target Species Goat | |
Concentration 1.5 mg active conjugate/ml | |
Conjugate Biotinylated | |
Reactive Species Rabbit | |
Source Species Goat | |
Host Species Rabbit |
Rabbit Anti-Goat IgG Antibody (H+L), Biotinylated
The biotinylated rabbit anti-goat IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
• Recognizes both heavy and light chains (H+L)
• Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
• Included in the VECTASTAIN® ABC kits
• Supplied in solution
• Suitable for use with goat, sheep, and bovine IgG primary antibodies
$206.00
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Technical Information Vector Laboratories affinity-purified antibodies are are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. With some exceptions the recommended dilution for most applications is 1:200. The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended. |
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