Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.
Biotinylated tomato lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
|Unit Size||1 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology|
|Recommended Usage||For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.|
|Recommended Storage||2-8 °C; Store frozen for long term storage|
|Solution||10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2|
|Concentration||2 mg active conjugate/ml|
|Sugar Specificity||[GlcNAc]1-3, N-Acetylglucosamine|
The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: Chitin Hydrolysate
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